Q 33E
Question
Question: What amino acid sequence is coded for by the following DNA coding strand (sense strand)? (5') CTT-CGA-CCA-GAC-AGC-TTT (3’)
Step-by-Step Solution
Verified The amino acid sequence for the given DNA (5') CTT-CGA-CCA-GAC-AGC-TTT (3’) is:
Leu-Arg-Pro-Asp-Ser-Phe.
Although DNA is double-stranded, only one strand is used as a transcription template at any given moment. The noncoding strand is the name given to this template strand. Because its sequence will be identical to that of the new RNA molecule, the non-template strand is referred to as the coding strand.
From the question, the DNA (sense strand) sequence is:
(5') CTT-CGA-CCA-GAC-AGC-TTT (32’)
Original DNA strand (sense Strand):
(5') CTT-CGA-CCA-GAC-AGC-TTT (32’)
The first step is to transform the DNA sense strand to its antisense strand.
Complementary DNA (antisense strand):
(3') CTT-CGA-CCA-GAC-AGC-TTT (5’)
The second step is to locate the mRNA by changing G with C, C with G, A with U, and T with A.
(5') CUU-CGA-CCA-GAC-AGC-UUU (3’)
As a result, the amino acid sequence will be:
Leu-Arg-Pro-Asp-Ser-Phe
The message now indicates that the first base is C, the second base is U, and the third base is U. From the code assignment table, select "Leu". The first base in (CGA) is C, the second base is G, and the third base is A. As a result, the message reads "Arg."
The first base is C, the second base is C, and the third base is A in (CCA). As a result, the message reads "Pro." Now (GAC), G is the first base, A is the second base, and C is the third base. As a result, the message reads "Asp".
The first base in (AGC) is A, the second base is G, and the third base is C. As a result, the notification reads "Ser". The final codon message is UUU, which stands for "Phe"
Leu-Arg-Pro-Asp-Ser-Phe is the final amino acid sequence obtained. It is also possible to produce mRNA directly during translation, which is a copy of the DNA coding strand with each T replaced by a U.