Q29-32E - Organic Chemistry (Mcmurry)

Question

Give an mRNA sequence that will code for the synthesis of angiotensin II.

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe

Step-by-Step Solution

Answer

The mRNA sequence that will code for the synthesis of angiotensin II is,

Asp	Arg	Val	Tyr	Ile	His	Pro	Phe
GAU	CGU	GUU	UAU	AUU	CAU	CCU	UUU
GAC	CGC	GUC	UAC	AUC	CAC	CCC	UUC
	CGA	GUA		AUA		CCA
	CGG	GUG				CCG

Step 1: Introduction

Messenger RNA (mRNA) is responsible for directing the creation of hundreds of different peptides and proteins that organisms require. Protein biosynthesis is carried out on ribosomes, which are tiny granular particles made up 60% of ribosomal RNA and 40% protein that are found in the cytoplasm of a cell.

The unique sequence of mRNA creates a message that specifies the amino acid residue sequences that must be linked, referred to as "codon." Each "word," or codon, is made up of three ribonucleotides that are unique to a certain amino acid. In a process known as translation, the message encoded in mRNA is read by transfer RNA (tRNA).

Step 2: Codon assignment of base triplet

The direction of writing the bounded amino acid sequence is always the same . tRNA, which possesses the corresponding anticodon base, reads the codon sequence on mRNA.

The tabulation is

Step 3: Sequence of codon

"Asp" has a codon message (GAU and GAC). The same may be said with "Arg" (CGU, CGC, CGA and CGG). Similarly, the message for "Val" will be as shown in the table (GUU, GUC, GUA and UUC).

The message will be the same for "Tyr" (UAU and UAC). The message for "Ile" is (AUU, AUC and AUA). It will be "His" message (CAU and CAC). The codon will be "Pro" for "Pro" (CCU, CCC, CCA and CCG). Finally, it would code as "Phe" for (UUU and UUC).

Step 4: Tabulation for the amino acid codon

The possible codon according to base triplet for given mRNA sequence is,

Asp	Arg	Val	Tyr	Ile	His	Pro	Phe
GAU	CGU	GUU	UAU	AUU	CAU	CCU	UUU
GAC	CGC	GUC	UAC	AUC	CAC	CCC	UUC
	CGA	GUA		AUA		CCA
	CGG	GUG				CCG