Chapter 9

Which of the following is LEAST likely to affect the purification of genomic DNA for analysis in molecular methods? a. Sample volume required for hybridization b. Size of the nucleic acid to be isolated c. Presence of RNA in the sample d. Presence of protein in the sample e. Presence of deoxyribose

Why is it important to add RNase to the patient sample when isolating genomic DNA? a. RNase serves as an enzymatic tag on DNA probes. b. RNase will remove excess collagen from the patient sample. c. RNase will transport the amino acid to the ribosome, where the peptide chain elongates. d. RNase clones DNA to RNA. e. RNase digests RNA in the patient sample that could copurify with genomic DNA.

All of the following describe a nucleic acid probe except: a. Short strand of DNA of a known sequence b. Short strand of RNA of a known sequence c. Can be cloned in bacteria d. Can be inserted into a plasmid vector e. Can be labeled with avidin