Chapter 15

Which of the following methods for lipoprotein electrophoresis depends on charge and molecular size? a. Polyacrylamide gel b. Paper c. Cellulose acetate d. Agarose

Which of the following statements concerning chylomicrons is FALSE? a. The major lipid transported by this lipoprotein is cholesterol. b. This lipoprotein is produced in the intestinal mucosa. c. The primary function is to carry dietary (exogenous) lipids to the liver. d. It remains at the origin (point of application) during lipoprotein electrophoresis.

The lipoprotein that contains the greatest amount of protein is called a. HDL b. Chylomicrons C. VLDL d. LDL

True or False? Pre-beta (VLDL)-lipoproteins migrate further toward the anode on polyacrylamide gel than they do on cellulose acetate or agarose.

Several enzymatic triglyceride methods measure the production or consumption of a. \(\mathrm{NADH}\) b. Fatty acids c. Glycerol d. Diacetyl lutidine

The most likely cause for serum/plasma to appear "milky" is the presence of a. Chylomicrons b. VLDL c. LDL d. HDL

In the colorimetric determination of cholesterol using the enzyme cholesterol oxidase, the agent that oxidizes the colorless organic compound \(4-\) aminoantipyrine to a pink complex is a. Hydrogen peroxide b. Cholest-4-ene-3-one c. NAD d. Phenol

Which lipoprotein is the major carrier of cholesterol to peripheral tissue? a. LDL b. Chylomicrons c. VLDL d. HDL

A patient is admitted to the hospital with intense chest pains. The patient's primary care physician requests the emergency department doctor to order several tests, including a lipid profile with cholesterol fractionation. Given the patient's results provided below, what would be the LDL-C for this patient? Total cholesterol \(=400 \mathrm{mg} / \mathrm{dL} ;\) triglycerides \(=300 \mathrm{mg} / \mathrm{dL} ; \mathrm{HDL}-\mathrm{C}=100\) \(\mathrm{mg} / \mathrm{dL}\); LP electrophoresis, pending. a. \(240 \mathrm{mg} / \mathrm{dL}\) b. \(160 \mathrm{mg} / \mathrm{dL}\) c. \(200 \mathrm{mg} / \mathrm{dL}\) d. \(300 \mathrm{mg} / \mathrm{dL}\)

As part of a lipoprotein phenotyping, it is necessary to perform total cholesterol and triglyceride determinations, as well as lipoprotein electrophoresis. The test results obtained from such studies were \- Triglyceride, \(340 \mathrm{mg} / \mathrm{dL}\) (reference range, \(<150 \mathrm{mg} / \mathrm{dL}\) ) \- Total cholesterol, \(180 \mathrm{mg} / \mathrm{dL}\) (reference range, \(<200 \mathrm{mg} / \mathrm{dL}\) ) \- Pre-beta-lipoprotein fraction increased \- Beta-lipoprotein fraction normal \- No chylomicrons present \- Serum appearance turbid The best explanation for these results would be that the patient exhibits a phenotype indicative of a. Type IV hyperlipoproteinemia b. Type I hyperlipoproteinemia c. Type II hyperlipoproteinemia d. Type III hyperlipoproteinemia e. Type V hyperlipoproteinemia

Which of the following results is the most consistent with high risk of CHD? a. \(20 \mathrm{mg} / \mathrm{dL} \mathrm{HDL}-\mathrm{C}\) and \(250 \mathrm{mg} / \mathrm{dL}\) total cholesterol b. \(35 \mathrm{mg} / \mathrm{dL}\) HDL-C and \(200 \mathrm{mg} / \mathrm{dL}\) total cholesterol c. \(50 \mathrm{mg} / \mathrm{dL} \mathrm{HDL}-\mathrm{C}\) and \(190 \mathrm{mg} / \mathrm{dL}\) total cholesterol d. \(55 \mathrm{mg} / \mathrm{dL} \mathrm{HDL}-\mathrm{C}\) and \(180 \mathrm{mg} / \mathrm{dL}\) total cholesterol e. \(60 \mathrm{mg} / \mathrm{dL}\) HDL-C and \(170 \mathrm{mg} / \mathrm{dL}\) total cholesterol

What is the presumed defect in most cases of familial type IIa hyperlipoproteinemia? a. Defective receptors for LDL b. Deficiency of hydroxymethylglutaryl (HMG)-CoA reductase c. Deficiency of cholesterol esterase d. Deficiency of LPL e. Defective esterifying enzymes LCAT and ACAT

Hyperchylomicronemia (type I) in childhood has been associated with which of the following? a. A deficiency of apo C-II b. A deficiency of LCAT c. A deficiency of LPL d. A deficiency of apo A-I