Chapter 11
Brock Biology of Microorganisms · 7 exercises
Problem 1
What are restriction enzymes? Why does the presence of a restriction enzyme in a cell not cause the degradation of that cell's DNA? (Section 11.1)
4 step solution
Problem 3
Describe the basic principles of gene amplification using the polymerase chain reaction (PCR). How have thermophilic and hyperthermophilic prokaryotes simplified the use of PCR? (Section 11.3)
5 step solution
Problem 4
Genetic engineering depends on vectors. Describe the properties needed in a well-designed plasmid cloning vector. (Section 11.4)
6 step solution
Problem 5
How could you detect a colony containing a cloned gene if you did not know the gene sequence but had available purified protein encoded by the gene? (Section 11.4)
6 step solution
Problem 7
What does site-directed mutagenesis allow you to do that normal mutagenesis does not? (Section 11.5)
4 step solution
Problem 12
Describe the similarities and differences between expression vectors and shuttle vectors. (Section 11.9)
4 step solution
Problem 13
How has bacteriophage T7 been used in expressing foreign genes in Escherichia coli, and what desirable features does this regulatory system possess? (Section 11.9)
4 step solution