Chapter 10

_______ cut(s) DNA molecules at specific sites. a. DNA polymerase b. DNA probes c. Restriction enzymes d. DNA ligase

A _____ is a small circle of bacterial DNA that is separate from the bacterial chromosome. a. plasmid b. chromosome c. nucleus d. double helix

For each species, all____ in the complete set of chromosomes is/are the _____ . a. genomes; genotype b. DNA; genome c. SNPs; DNA profile d. DNA; library

A set of cells that host various DNA fragments collectively representing an organism's entire set of genetic information is a_____ . a. genome b. clone c. DNA library d. GMO

PCR can be used to ____ . a. increase the number of specific DNA fragments b. check DNA fingerprints c. modify a human genome d. a and b are correct

Fragments of DNA can be separated by electrophoresis according to _____ . a. sequence b. length c. species

Taq polymerase is used for PCR because it _____ . a. tolerates the high temperature needed to separate DNA strands b. is an enzyme from a bacterium c. does not require primers

______ is a technique to determine the order of nucleotides in a fragment of DNA. a. \(\mathrm{PCR}\) b. Sequencing c. Electrophoresis d. Nucleic acid hybridization

Which of the following can be used to carry foreign DNA into host cells? Choose all correct answers. .a. RNA b. viruses c. PCR d. plasmids e. lipid clusters f. blasts of microscopic pellets g. xenotransplantation h. sequencing

A transgenic organism a. carries a gene from another species b. has been genetically modified c. can pass a foreign gene to offspring d. all of the above

______ can correct a genetic defect in an individual. a. Cloning vectors b. Gene therapy c. Xenotransplantation d. a and b

Put the following tasks in the order they would occur during a cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. using DNA polymerase to sequence the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymes

Match the terms with the best description. _______DNA profile _______ Ti plasmid _______nucleic acid _______hybridization _______ eugenics _______ SNP _______transgenic _______GMO a. GMO with a foreign gene b. alleles commonly contain them c. a person's unique collection of short tandem repeats d. base pairing of DNA or DNA and RNA from different sources e. selecting "desirable" traits f. genetically modified g. used in some gene transfers