Chapter 14

A plasmid (a) is used as a DNA vector (b) is a type of bacteriophage \((c)\) is a type of \(\operatorname{cDNA}(d)\) is a retrovirus (e) b and c.

DNA molecules with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages.

Human DNA and a particular plasmid both have sites that are cut by the restriction enzymes HindIII and EcoRI. To make recombinant DNA, the scientist should (a) cut the plasmid with EcoRI and the human DNA with HindIII (b) use EcoRI to cut both the plasmid and the human DNA (c) use HindIII to cut both the plasmid and the human DNA (d) a or b (e) b or c.

The PCR technique uses (a) heat-resistant DNA polymerase (b) reverse transcriptase (c) DNA ligase (d) restriction enzymes (c) b and \(c\).

A cDNA clone contains (a) introns (b) exons (c) anticodons (d) a and b (e) b and c.

The dideoxynucleotides ddATP, ddTTP, ddGTP, and ddCTP are important in DNA sequencing because they (a) cause premature termination of a growing DNA strand (b) are used as primers (c) cause the DNA fragments that contain them to migrate more slowly through a sequencing gel (d) are not affected by high temperatures (e) have more energy than deoxynucleotides.

In the Southern blot technique, ______________ is/are transferred from a gel to a nitrocellulose or nylon membrane. (a) protein (b) RNA (c) DNA (d) bacterial colonies (e) reverse transcriptase.

Gel electrophoresis separates nudeic acids on the basis of differences in (a) length (molecular weight) (b) charge (c) nucleotide sequence (d) relative proportions of adenine and guanine (e) relative proportions of thymine and cytosine.

The Ti plasmid, carried by Agrobacterium tumefaciens, is especially useful for introducing genes into (a) bacteria (b) plants (c) animals (d) yeast (e) all cukaryotes.

A genomic library (a) represents all the DNA in a specific chromosome (b) is made using reverse transcriptase (c) is stored in a collection of recombinant bacteria (d) is a DNA copy of mature mRNAs (e) allows researchers to amplify a tiny sample of DNA.

Tissue growth factor-beta (a) is a genetic probe for recombinant plasmids (b) is a product of DNA technology used in tissue engineering (c) is necessary to make a cDNA library (d) cannot be synthesized without a heat-resistant DNA polymerase (e) is isolated by the Southern blot technique

These highly polymorphic molecular markers are useful in DNA typing (a) short tandem repeats (b) cloned DNA sequences (c) palindromic DNA sequences (d) cosmid cloning vectors (c) complementary DNAs.