Q.21
Question
The advantage of yeast cells over bacterial cells to express human proteins is that:
a. yeast cells grow faster
b. yeast cells are easier to manipulate genetically
c. yeast cells are eukaryotic and modify proteins similarly to human cells
d. yeast cells are easily lysed to purify the proteins
Step-by-Step Solution
VerifiedThe correct answer is (c) yeast cells are eukaryotic and modify proteins similarly to human cells.
- Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom.
- Yeasts have two main uses in food production: baking and making alcoholic beverages.
- Yeasts are known as facultative anaerobes. Facultative anaerobes can survive in both aerobic and anaerobic conditions.
c. yeast cells are eukaryotic and modify proteins similarly to human cells
As a eukaryotic organism, the yeast cell produces and modifies proteins in a manner similar to human cells, as opposed to the bacterium Escherichia coli, which lacks the internal membrane structures and enzymes to tag proteins for export.
a. yeast cells grow faster
Each yeast cell can bud 20-30 times during its life, on each occasion producing an identical new cell that can make the same number of new cells again. In favorable conditions, multiplication can be very fast.
b. yeast cells are easier to manipulate genetically
Yeast cells share many basic biological properties with our cells. Genetic manipulation in yeast is easy and cheap. Yeast also grows quickly.
Yeast cells are a good model organism because you can grow a culture overnight.
d. yeast cells are easily lysed to purify the proteins
The Yeast Lysis Buffer is useful for extraction of soluble proteins from yeast cells. As a result, extracted proteins become degraded or artifactually. modified