Q. 17.73 - An Introduction to General, Organic, and Biological Chemistry

Question

How is a gene for a particular protein inserted into a plasmid?

Step-by-Step Solution

Answer

The gene of particular interest is inserted and cleaved from the source to the organism by enzymes known as restriction enzymes, and after the process, the DNA insert and plasmid are constructed.

Step 1: Given Information

We have to find out the process of a gene being inserted into a plasmid.

Step 2: Explanation

The gene that has to be inserted into the plasmid is called a DNA insert. The DNA insert is first excised from the organism's genomic source using an enzyme known as a restriction enzyme.

Circular plasmids are cleaved using the same or different restriction enzymes as needed. The DNA insert is now glued with an effective glue enzyme called DNA ligase and that's how the whole process of inserting gene into plasmid is carried out.